Automation of C-terminal sequence analysis of 2D-PAGE separated proteins
نویسندگان
چکیده
منابع مشابه
Sequential analysis of N- and O-linked glycosylation of 2D-PAGE separated glycoproteins.
A robust method has been developed that allows analysis of both N- and O-linked oligosaccharides released from glycoproteins separated using 2D-PAGE and then electroblotted to PVDF membrane. This analysis provides efficient oligosaccharide profiling applicable to glycoproteomic analysis. The method involves the enzymatic release of N-linked oligosaccharides using PNGase F followed by the chemic...
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ژورنال
عنوان ژورنال: EuPA Open Proteomics
سال: 2014
ISSN: 2212-9685
DOI: 10.1016/j.euprot.2014.03.004